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RB-05-0003B-50
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RayBiotech, Inc.
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Recombinant anti-C. botulinum Toxin C with anti-Myc Tag [+Biotin]
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RB-05-0003 specifically detects Toxin C as determined from testing bacterial samples in Dot Blot and ELISA assays.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml). Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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RB-05-0008B-50
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RayBiotech, Inc.
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Human anti-Escherichia Coli O157:H7 with anti-Myc tag [+Biotin]
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It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml), (see image)The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.. Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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130-00030B-50
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RayBiotech, Inc.
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Mouse anti-TIMP-3 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 0.5 �g/ml), Western Blotting (recommended work dilution= 1-2 �g/ml), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, protein BSA, Lane 3, recombinant protein TIMP1. The membrane blot was probed with anti-Timp1 primary antibody (1.5 �g/ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10058B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 15.6 �g/ml), Western Blotting (recommended work dilution= 1: 1,000(at least)), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody(1?g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10059B-50
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RayBiotech, Inc.
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Mouse anti-VEGF [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein VEGF, Lane 2, protein marker. The membrane blot was probed with antiVEGF primary antibody ( 0.5 �g/ ml ). Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10060B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10063B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
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130-10071B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10082B-50
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RayBiotech, Inc.
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Mouse anti-AR [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 12.5 �g/ml ), Western Blotting (recommended work dilution= 1:2,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein BSA, Lane 2, recombinant protein AR, Lane 3, protein marker. The membrane blot was probed with anti-AR primary antibody (0.5 �g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10083B-50
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RayBiotech, Inc.
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Mouse anti-AR [+Biotin], Mouse Monoclonal Antibody
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ELISA: Recommended to dilute to ~6.25 µ,g/ml, Western Blot: Recommended dilution= 1:2000, Immunodetection Analysis (see image below),
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130-10086B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10096B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system. Lane 1 and 4. Lysates from two selected MRSA isolated strains, Lane 2-3. Lysates from two selected MSSA strains.
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130-10151B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: First Antibody: 1ug/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 5000X (HRP-Goat Anti-Mouse IgM) M: Protein Marker Lane 1: Recombinant PBP2a 1ug Lane 2: MSSA lysate 15ul
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130-10178B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1:1,000-5,000),see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10179B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:40,000), Western Blotting (recommended work dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10305B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5C6-E11-C2-G11-D2) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10306B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (4D2-E1-D5-G8-G11-E11) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10307B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5B9-G2-F12) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006B-50
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RayBiotech, Inc.
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Mouse anti-TNF alpha [+Biotin], Mouse Monoclonal Antibody
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha.,
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RB-10-0004B-50
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RayBiotech, Inc.
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Mouse anti-E-tag [+Biotin], Mouse Monoclonal Antibody
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E-Tag antibody detects fusion recombinant antibodies derived from a variety of sources at various concentrations. No cross reactivity with other-tags-fusion protein was found in ELISA or Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1:1,000
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RB-10-0007B-50
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RayBiotech, Inc.
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Mouse anti-Human-C-myc-Tag [+Biotin], Mouse Monoclonal Antibody
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Myc-Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No cross reactivity was found with other epitope tags in ELISA or Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1-2 �g/ml)
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RB-14-0001B-50
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RayBiotech, Inc.
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Mouse anti-B2M [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-2000(at least)), Immunohistology-frozen, Apoptosis Induction (recommended work dilution= study in vitro and vivo), (see image)Immunodetection Analysis: The membrane blot was probed with anti- beta2 M primary antibody(1 �g/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular recombinant beta2 M (12 kDa).
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RB-IP-10-101B-50
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RayBiotech, Inc.
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Mouse anti-HA-Tag [+Biotin], Mouse Monoclonal Antibody
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HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
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130-00016B-50
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RayBiotech, Inc.
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TIMP1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 64,000), Western Blotting (recommended work dilution= 1:1,000), (see image) Western blot analysis of recombinant human TIMP-1, using anti-TIMP-1 antibody (1 : 1,000)
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