Applications: | Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha., |
Species Reactivity: | Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested. |
Isotype: | IgG1 |
Conjugation: | Biotin |
Description: | Mouse anti-TNF alpha Biotinylated Antibody, 50 ug |
Ascension Number: | NM_000595 |
Shipping Conditions: | Ship on cold packs |
Storage: | The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles. |
Usage: | Research use only |