Applications: | ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system. |