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RB-05-0003B-50
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RayBiotech, Inc.
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Recombinant anti-C. botulinum Toxin C with anti-Myc Tag [+Biotin]
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RB-05-0003 specifically detects Toxin C as determined from testing bacterial samples in Dot Blot and ELISA assays.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml). Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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RB-05-0008B-50
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RayBiotech, Inc.
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Human anti-Escherichia Coli O157:H7 with anti-Myc tag [+Biotin]
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It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml), (see image)The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.. Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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127-10128
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RayBiotech, Inc.
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Mouse anti-Procalcitonin [+Biotin], Mouse Monoclonal Antibody
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Detection of human procalcitonin in ELISA and Western Blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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130-00030B-50
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RayBiotech, Inc.
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Mouse anti-TIMP-3 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 0.5 �g/ml), Western Blotting (recommended work dilution= 1-2 �g/ml), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, protein BSA, Lane 3, recombinant protein TIMP1. The membrane blot was probed with anti-Timp1 primary antibody (1.5 �g/ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10058B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 15.6 �g/ml), Western Blotting (recommended work dilution= 1: 1,000(at least)), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody(1?g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10059B-50
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RayBiotech, Inc.
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Mouse anti-VEGF [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein VEGF, Lane 2, protein marker. The membrane blot was probed with antiVEGF primary antibody ( 0.5 �g/ ml ). Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10060B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10063B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
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130-10071B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10082B-50
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RayBiotech, Inc.
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Mouse anti-AR [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 12.5 �g/ml ), Western Blotting (recommended work dilution= 1:2,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein BSA, Lane 2, recombinant protein AR, Lane 3, protein marker. The membrane blot was probed with anti-AR primary antibody (0.5 �g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10083B-50
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RayBiotech, Inc.
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Mouse anti-AR [+Biotin], Mouse Monoclonal Antibody
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ELISA: Recommended to dilute to ~6.25 µ,g/ml, Western Blot: Recommended dilution= 1:2000, Immunodetection Analysis (see image below),
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130-10086B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10096B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system. Lane 1 and 4. Lysates from two selected MRSA isolated strains, Lane 2-3. Lysates from two selected MSSA strains.
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130-10151B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: First Antibody: 1ug/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 5000X (HRP-Goat Anti-Mouse IgM) M: Protein Marker Lane 1: Recombinant PBP2a 1ug Lane 2: MSSA lysate 15ul
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130-10178B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1:1,000-5,000),see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10179B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:40,000), Western Blotting (recommended work dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10305B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5C6-E11-C2-G11-D2) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10306B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (4D2-E1-D5-G8-G11-E11) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10307B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5B9-G2-F12) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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136-01136-100
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RayBiotech, Inc.
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Anti-Human alpha-1-Antitrypsin Antibody, Biotinylated, Mouse Monoclonal Antibody
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Human
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Flow Cytometry, Western Blot, Immunohistochemistry; Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006B-50
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RayBiotech, Inc.
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Mouse anti-TNF alpha [+Biotin], Mouse Monoclonal Antibody
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha.,
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RB-10-0004B-50
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RayBiotech, Inc.
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Mouse anti-E-tag [+Biotin], Mouse Monoclonal Antibody
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E-Tag antibody detects fusion recombinant antibodies derived from a variety of sources at various concentrations. No cross reactivity with other-tags-fusion protein was found in ELISA or Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1:1,000
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RB-10-0007B-50
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RayBiotech, Inc.
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Mouse anti-Human-C-myc-Tag [+Biotin], Mouse Monoclonal Antibody
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Myc-Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No cross reactivity was found with other epitope tags in ELISA or Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting (recommended work dilution= 1-2 �g/ml)
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RB-14-0001B-50
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RayBiotech, Inc.
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Mouse anti-B2M [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-2000(at least)), Immunohistology-frozen, Apoptosis Induction (recommended work dilution= study in vitro and vivo), (see image)Immunodetection Analysis: The membrane blot was probed with anti- beta2 M primary antibody(1 �g/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular recombinant beta2 M (12 kDa).
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