|
130-00030B-50
|
RayBiotech, Inc.
|
Mouse anti-TIMP-3 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= at least detecting 0.5 �g/ml), Western Blotting (recommended work dilution= 1-2 �g/ml), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, protein BSA, Lane 3, recombinant protein TIMP1. The membrane blot was probed with anti-Timp1 primary antibody (1.5 �g/ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
130-10058B-50
|
RayBiotech, Inc.
|
Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= at least detecting 15.6 �g/ml), Western Blotting (recommended work dilution= 1: 1,000(at least)), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody(1?g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
130-10059B-50
|
RayBiotech, Inc.
|
Mouse anti-VEGF [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein VEGF, Lane 2, protein marker. The membrane blot was probed with antiVEGF primary antibody ( 0.5 �g/ ml ). Proteins were visualized using a donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
130-10060B-50
|
RayBiotech, Inc.
|
Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
130-10063B-50
|
RayBiotech, Inc.
|
Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
|
|
|
130-10071B-50
|
RayBiotech, Inc.
|
Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
130-10082B-50
|
RayBiotech, Inc.
|
Mouse anti-AR [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 12.5 �g/ml ), Western Blotting (recommended work dilution= 1:2,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein BSA, Lane 2, recombinant protein AR, Lane 3, protein marker. The membrane blot was probed with anti-AR primary antibody (0.5 �g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
130-10086B-50
|
RayBiotech, Inc.
|
Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
|
|
|
RB-01-0001B-50
|
RayBiotech, Inc.
|
Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
|
|
ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
|
|
|
RB-08-0003B-50
|
RayBiotech, Inc.
|
Visfatin [+Biotin], Rabbit Polyclonal Antibody
|
|
ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 �g/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
|
|
|
RB-08-0013B-50
|
RayBiotech, Inc.
|
POMC [+Biotin], Rabbit Polyclonal Antibody
|
|
ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
|
|
