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RB-05-0008B-50
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RayBiotech, Inc.
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Human anti-Escherichia Coli O157:H7 with anti-Myc tag [+Biotin]
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It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml), (see image)The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.. Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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130-00030B-50
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RayBiotech, Inc.
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Mouse anti-TIMP-3 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 0.5 �g/ml), Western Blotting (recommended work dilution= 1-2 �g/ml), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, protein BSA, Lane 3, recombinant protein TIMP1. The membrane blot was probed with anti-Timp1 primary antibody (1.5 �g/ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10058B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 15.6 �g/ml), Western Blotting (recommended work dilution= 1: 1,000(at least)), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody(1?g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10060B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10063B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
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130-10071B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10082B-50
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RayBiotech, Inc.
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Mouse anti-AR [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 12.5 �g/ml ), Western Blotting (recommended work dilution= 1:2,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein BSA, Lane 2, recombinant protein AR, Lane 3, protein marker. The membrane blot was probed with anti-AR primary antibody (0.5 �g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10086B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10096B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system. Lane 1 and 4. Lysates from two selected MRSA isolated strains, Lane 2-3. Lysates from two selected MSSA strains.
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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130-10000B-50
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RayBiotech, Inc.
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Androgen Receptor [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa).
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130-10008B-50
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RayBiotech, Inc.
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ESR1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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130-10062B-50
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RayBiotech, Inc.
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CKB [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000), Western Blotting (recommended work dilution= 1: 1,000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa).
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130-10068B-50
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RayBiotech, Inc.
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MB [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MB, Lane 2, mouse heart cell lysate, Lane 3, mouse muscle cell lysate. The membrane blot was probed with antiMB primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Mouse cells (17 kDa).
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130-10147B-50
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RayBiotech, Inc.
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Trefoil factor 3 (TFF3) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 8,000), Western Blotting (recommended work dilution= 1:1000-5,000) , (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein Inhibin alpha. The membrane blot was probed with anti- Inhibin alpha primary antibody (0.2 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein Inhibin alpha from E.coli cell (14kDa).
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RB-02-0004B-50
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RayBiotech, Inc.
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EGFR/ErbB1 (pSer1045) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 5,000-10,000), Western Blotting (recommended work dilution= 1-5 �g/ml), (see image) RB-02-0002 (1-5 �g/ml) staining of Hela and A431 lysate. Lane 1 and 2, Hela lysate with and without stimulating, Land 3 and 4, A431 lysate with and without stimulating. Detected by western blot using chemiluminescence.
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RB-08-0003B-50
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RayBiotech, Inc.
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Visfatin [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100 �g/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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RB-08-0013B-50
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RayBiotech, Inc.
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POMC [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
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