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188-10024-1
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RayBiotech, Inc.
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Rabbit anti-CD3e Antibody
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Human, Mouse, Rat, Primate, Guinea Pig, Hamster, Horse, Mole Rat, Pig, Rabbit, Sheep, Cat, Chicken, Cow, Dog, Frog, Opossum, Elephant. Others species not known.
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FACS: use the CD3e antibody at 0.5-1ug/million cells<br/>WB: 0.5-1.0ug/ml<br/>IP: 1-2ug/500ug protein lysate,
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188-10024-SAF
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RayBiotech, Inc.
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Rabbit anti-CD3e Antibody [Sodium Azide Free]
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Human, Mouse, Rat, Primate, Guinea Pig, Hamster, Horse, Mole Rat, Pig, Rabbit, Sheep, Cat, Chicken, Cow, Dog, Frog, Opossum, Elephant. Others species not known.
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FACS: use the CD3e antibody at 0.5-1ug/million cells<br/>WB: 0.5-1.0ug/ml<br/>IP: 1-2ug/500ug protein lysate,
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188-10184-1
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RayBiotech, Inc.
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Rabbit anti-Human/Mouse/Rat/Horse/Cow/Dog TIMP3 Antibody
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Human, Mouse, Rat, Horse, Cow and Dog. Other species not known.
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FACS: use antibody at 0.5-1ug/million cells<br/>IF: 1-2ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 1-2ug/500ug protein lysate<br/>Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
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188-10184-SAF
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RayBiotech, Inc.
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Rabbit anti-Human/Mouse/Rat/Horse/Cow/Dog TIMP3 Antibody [Sodium Azide Free]
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Human, Mouse, Rat, Horse, Cow and Dog. Other species not known.
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FACS: use antibody at 0.5-1ug/million cells<br/>IF: 1-2ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 1-2ug/500ug protein lysate<br/>Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
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188-11245-1
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RayBiotech, Inc.
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Rabbit anti-Human Recombinant von Willebrand Factor / vWFAntibody
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Human. Other species not known.
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Western blot: 1-2ug/ml<br/>Immunoprecipitation: 1-2ug/500ug lysate<br/>Immunofluorescence: 1-2ug/ml<br/>Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
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188-11245-SAF
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RayBiotech, Inc.
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Rabbit anti-Human Recombinant von Willebrand Factor / vWFAntibody [Sodium Azide Free]
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Human. Other species not known.
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Western blot: 1-2ug/ml<br/>Immunoprecipitation: 1-2ug/500ug lysate<br/>Immunofluorescence: 1-2ug/ml<br/>Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
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RB-01-0001-100
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RayBiotech, Inc.
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Mouse anti-Human bFGF/FGF basic/FGF2
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006-100
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RayBiotech, Inc.
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Mouse anti-Human TNF alpha
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 ng/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 ?g/mL with the appropriate secondary reagents to detect human TNF-alpha.,
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RB-02-0004-100
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RayBiotech, Inc.
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Rabbit anti-Human EGFR/ErbB1 (pSer1045)
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ELISA (recommended work dilution= 1: 5,000-10,000), Western Blotting (recommended work dilution= 1-5 �g/ml), (see image) RB-02-0002 (1-5 �g/ml) staining of Hela and A431 lysate. Lane 1 and 2, Hela lysate with and without stimulating, Land 3 and 4, A431 lysate with and without stimulating. Detected by western blot using chemiluminescence.
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RB-08-0003-100
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RayBiotech, Inc.
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Rabbit anti-Human Visfatin
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ELISA (recommended work dilution= 1:10e6(at least detecting 10ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100pg/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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RB-08-0004-100
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RayBiotech, Inc.
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Rabbit anti-Human Apelin ligand (N-terminus)
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Human Apelin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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(under testing)Direct ELISA: antibody detection limit dilution will be 1:10,000-20,000. Western Blot: This antibody will be used at 1 - 2 �g/mL with the appropriate secondary reagents to detect human Apelin. Immunohistochemistry: under testing
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RB-08-0007-100
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RayBiotech, Inc.
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Rabbit anti-Human Amylin
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Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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ELISA (recommended work dilution= 1: 5,000), Western Blotting (recommended work dilution= 1: 1,000)
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RB-08-0013-100
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RayBiotech, Inc.
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Rabbit anti-Human POMC
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ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
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130-10058B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= at least detecting 15.6 �g/ml), Western Blotting (recommended work dilution= 1: 1,000(at least)), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody(1?g/ ml). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10060B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10063B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
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130-10071B-50
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RayBiotech, Inc.
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Mouse anti-IL-6 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 15.6 �g/ml ), Western Blotting (recommended work dilution= 1:1,000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IL-6, Lane 3, 293T lysate, Lane 4, SP2/0 lysate. The membrane blot was probed with anti-IL-6 primary antibody (1 �g/ ml). Proteins were visualized using a Donkey antimouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10086B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, SP2/0 lysate. The blot was probed with anti-IGF-1primary antibody (1:1,000). Proteins were visualized using a Donkey anti-mouse secondary antibody conjugated to IRDye 800CW detection system.
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130-10151B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: First Antibody: 1ug/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 5000X (HRP-Goat Anti-Mouse IgM) M: Protein Marker Lane 1: Recombinant PBP2a 1ug Lane 2: MSSA lysate 15ul
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130-10178B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1:1,000-5,000),see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10179B-50
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RayBiotech, Inc.
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Mouse anti-Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:40,000), Western Blotting (recommended work dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10305B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5C6-E11-C2-G11-D2) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10306B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (4D2-E1-D5-G8-G11-E11) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000),(see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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130-10307B-50
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RayBiotech, Inc.
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Mouse anti-PBP2a (5B9-G2-F12) [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
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188-10007-1
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RayBiotech, Inc.
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Bcl-2 Antibody, Mouse Monoclonal Antibody
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Human. Does not react with mouse and rat. Other species not known.
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ELISA: order BSA-free format for coating<br/>FACS: 0.5-1ug/million cells<br/>IF: 1-2ug/ml<br/>WB: 0.5-1ug/ml<br/>IP: 1-2ug/0.5mg lysate<br/>IHC (FFPE): 0.5-1ug/ml for 30 minutes at RT (1)<br/>Prediluted format: incubate for 30 min at RT (2)
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