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RB-05-0003B-50
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RayBiotech, Inc.
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Recombinant anti-C. botulinum Toxin C with anti-Myc Tag [+Biotin]
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RB-05-0003 specifically detects Toxin C as determined from testing bacterial samples in Dot Blot and ELISA assays.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml). Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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RB-05-0008B-50
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RayBiotech, Inc.
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Human anti-Escherichia Coli O157:H7 with anti-Myc tag [+Biotin]
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It specifically detects protein derived from testing bacterial sample at various concentrations. See image Below.
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ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 �g/ml), Dot Blotting(recommended work dilution= 1~5 �g/ml), (see image)The Purity and binding activity of recombinant antibody, anti-O157:H7 by Dot-blotting assay. A. O157 scFv binding activity. Directly spotting O157 antigen into membrane in Row I and II with two dilution (100 and 10 ng of each spot 2-3 ) plus positive system control in spotting blot 1 and 5 as well as BSA control in Spot 4, respectively, then probe the membrane by using O157:H7-specific recombinant antibody after blocking and following anti-Myc-tag and HRP-conjugated detection antibody. B. Checking purity of purified O157 scFv. Directly probed blocking blot with anti-Myctag and following HRP-conjugated detection antibodies.. Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
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127-10128
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RayBiotech, Inc.
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Mouse anti-Procalcitonin [+Biotin], Mouse Monoclonal Antibody
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Detection of human procalcitonin in ELISA and Western Blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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130-10063B-50
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RayBiotech, Inc.
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Mouse anti-IGF-1 [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 6.25 �g/ml ), Western Blotting (recommended work dilution= 1:2000 ), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, protein marker, Lane 2, recombinant protein IGF-1, Lane 3, 3T3-L-1 lysate. The membrane blot was probed with anti-IGF-1 primary antibody (1 �g/ ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system.
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130-10096B-50
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RayBiotech, Inc.
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Mouse anti-MRSA [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system. Lane 1 and 4. Lysates from two selected MRSA isolated strains, Lane 2-3. Lysates from two selected MSSA strains.
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136-01136-100
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RayBiotech, Inc.
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Anti-Human alpha-1-Antitrypsin Antibody, Biotinylated, Mouse Monoclonal Antibody
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Human
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Flow Cytometry, Western Blot, Immunohistochemistry; Each lot of antibody has been pre-titrated and tested by flow cytometry staining. We suggest use 5 µl per 106 cells in 100 µl volume of staining buffer.
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006B-50
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RayBiotech, Inc.
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Mouse anti-TNF alpha [+Biotin], Mouse Monoclonal Antibody
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha.,
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RB-14-0001B-50
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RayBiotech, Inc.
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Mouse anti-B2M [+Biotin], Mouse Monoclonal Antibody
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ELISA (recommended work dilution= 1:10e6(at least detecting 10 �g/ml)), Western Blotting (recommended work dilution= 1:1000-2000(at least)), Immunohistology-frozen, Apoptosis Induction (recommended work dilution= study in vitro and vivo), (see image)Immunodetection Analysis: The membrane blot was probed with anti- beta2 M primary antibody(1 �g/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular recombinant beta2 M (12 kDa).
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RB-IP-10-101B-50
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RayBiotech, Inc.
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Mouse anti-HA-Tag [+Biotin], Mouse Monoclonal Antibody
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HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.
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ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 �g/ml)
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130-10000B-50
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RayBiotech, Inc.
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Androgen Receptor [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa).
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130-10001B-50
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RayBiotech, Inc.
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TIMP1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. The membrane blot was probed with anti-TIMP1 primary antibody(0.25 �g/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular TIMP1 from human and mouse cells (23 kDa).
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130-10006B-50
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RayBiotech, Inc.
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TIMP1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:3.2x10e5(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein TIMP1. The membrane blot was probed with antiTIMP1 primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular TIMP1 from human and mouse cells (23 kDa).
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130-10008B-50
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RayBiotech, Inc.
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ESR1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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130-10009B-50
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RayBiotech, Inc.
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MIF [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein MIF, Lane 2.Jurkat cell lysate. The membrane blot was probed with anti-MIF primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MIF from human and mouse cells (20 kDa).
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130-10013B-50
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RayBiotech, Inc.
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CT1 [+Biotin], Rabbit Polyclonal Antibody
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Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
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ELISA (recommended work dilution= 1:800,000(at least detecting 12.5 �g/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25 �g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa).
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130-10015B-50
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RayBiotech, Inc.
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Pro-BNP [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 20,000(at least detecting 50 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa).
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130-10016B-50
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RayBiotech, Inc.
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MMP1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA: Dilution = 1:160,000 (at least detecting 6.25 ng/ml, Western Blotting: Dilution = 1:1,000-5,000, Immunodetection Analysis: Representative blot image below from a previous lot. The membrane blot was probed with antiMMP1 primary antibody (0.25 �g/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MMP1 from human and mouse cells (45 kDa).,
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130-10017B-50
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RayBiotech, Inc.
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MMP7 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:10,000(at least detecting 100 �g/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant proteinMMP7. The membrane blot was probed with anti-MMP7 primary antibody(0.25 �g/ml). Proteins werevisualized using a goat anti-rabbit secondary antibodyconjugated to HRP and chemiluminescence detectionsystem. Arrows indicate cellular MMP7 from humanand mouse cells (35 kDa).
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130-10020B-50
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RayBiotech, Inc.
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Der P1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 20,000(at least detecting 50 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. The membrane blot was probed with anti-Der P1 primary antibody (0.25 �g/ml). Recombinant proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate recombinant immunogen Der P1 (15kDa).
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130-10062B-50
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RayBiotech, Inc.
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CKB [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000), Western Blotting (recommended work dilution= 1: 1,000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa).
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130-10064B-50
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RayBiotech, Inc.
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MSLN [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1: 1,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MSLN. The membrane blot was probed with antiMSLN primary antibody (1 �g/ml). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular MSLN from human and mouse cells (34 kDa).
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130-10066B-50
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RayBiotech, Inc.
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TNi [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1:160,000), Western Blotting (recommended work dilution= 1: 1,000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein TnI. The membrane blot was probed with anti-TnI primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular TNi from human and mouse cells (27 kDa).
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RB-02-0004B-50
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RayBiotech, Inc.
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EGFR/ErbB1 (pSer1045) [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 5,000-10,000), Western Blotting (recommended work dilution= 1-5 �g/ml), (see image) RB-02-0002 (1-5 �g/ml) staining of Hela and A431 lysate. Lane 1 and 2, Hela lysate with and without stimulating, Land 3 and 4, A431 lysate with and without stimulating. Detected by western blot using chemiluminescence.
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RB-08-0002B-50
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RayBiotech, Inc.
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Vaspin [+Biotin], Rabbit Polyclonal Antibody
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Direct and indirect ELISA: antibody detection limit dilution at least 1:1,000-2,000. Western Blot: Reacted with the approx 48-kDa Vaspin His tagged protein. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature. Recommended for use at dilution 1:500-1,000.
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