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130-10000-100
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RayBiotech, Inc.
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Rabbit anti-Human Androgen Receptor
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa).
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130-10008-100
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RayBiotech, Inc.
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Rabbit anti-Human ESR1
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32ng/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2?g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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130-10009-100
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RayBiotech, Inc.
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Rabbit anti-Human MIF
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein MIF, Lane 2.Jurkat cell lysate. The membrane blot was probed with anti-MIF primary antibody(0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MIF from human and mouse cells (20 kDa).
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130-10013-100
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RayBiotech, Inc.
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Rabbit anti-Human CT1
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Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
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ELISA (recommended work dilution= 1:800,000(at least detecting 12.5 ng/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25?g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa).
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130-10015-100
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RayBiotech, Inc.
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Rabbit anti-Human Pro-BNP
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ELISA (recommended work dilution= 1: 20,000(at least detecting 50ng/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25?g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa).
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130-10062-100
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RayBiotech, Inc.
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Rabbit anti-Human CKB
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ELISA (recommended work dilution= 1: 40,000), Western Blotting (recommended work dilution= 1: 1,000), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1, Hela cell lysate, Lane 2, 293T cell lysate l, Lane 3, recombinant protein CKB. The membrane blot was probed with anti-CKB primary antibody. Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular CKB from human and mouse cells (43 kDa).
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130-10067-100
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RayBiotech, Inc.
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Rabbit anti-Human GPBB
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000), Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein GPBB, Lane 2, HepG2 cell lysate. The membrane blot was probed with anti-GPBB primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Human cells (95 kDa).
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130-10068-100
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RayBiotech, Inc.
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Rabbit anti-Human MB
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ELISA (recommended work dilution= 1: 80,000), Western Blotting (recommended work dilution= 1:1000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MB, Lane 2, mouse heart cell lysate, Lane 3, mouse muscle cell lysate. The membrane blot was probed with antiMB primary antibody (1 �g/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular from Mouse cells (17 kDa).
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RB-01-0001-100
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RayBiotech, Inc.
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Mouse anti-Human bFGF/FGF basic/FGF2
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006-100
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RayBiotech, Inc.
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Mouse anti-Human TNF alpha
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 ng/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 ?g/mL with the appropriate secondary reagents to detect human TNF-alpha.,
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RB-08-0003-100
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RayBiotech, Inc.
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Rabbit anti-Human Visfatin
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ELISA (recommended work dilution= 1:10e6(at least detecting 10ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Enzyme Immunoassay (EIA) (recommended work dilution= Sensitivity (100pg/ml)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3-4.Positive control, Hela and MD231 whole cell lysate, Lane 5.Negative control. The membrane blot was probed with antiVisfatin primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular Visfatin from human and mouse cells (52 kDa).
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RB-08-0004-100
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RayBiotech, Inc.
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Rabbit anti-Human Apelin ligand (N-terminus)
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Human Apelin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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(under testing)Direct ELISA: antibody detection limit dilution will be 1:10,000-20,000. Western Blot: This antibody will be used at 1 - 2 �g/mL with the appropriate secondary reagents to detect human Apelin. Immunohistochemistry: under testing
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RB-08-0007-100
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RayBiotech, Inc.
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Rabbit anti-Human Amylin
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Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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ELISA (recommended work dilution= 1: 5,000), Western Blotting (recommended work dilution= 1: 1,000)
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RB-08-0013-100
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RayBiotech, Inc.
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Rabbit anti-Human POMC
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ELISA (recommended work dilution= 1:10e5), Western Blotting (recommended work dilution= 1:1000-2000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2.Reference control, Lane 3. 3T3-L1 cell lysate, Lane 4. Hela cell lysate, Lane 5. NIH3T3 cell lysate. The membrane blot was probed with anti-POMC primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular POMC-associated protein from cells.
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188-10073-1
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RayBiotech, Inc.
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EGF Receptor Antibody, Mouse Monoclonal Antibody
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Human. Does not react with mouse and rat. Other species not known.
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Functional Activity (Inhibition): antibody blocks EGF receptor activation, inhibits A431 cell proliferation, arrests tumor growth in vivo, affinity purification<br/>IP: 1-2�g/500ug protein lysate<br/>FACS: 0.5-1ug/million cells
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188-10073-SAF
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RayBiotech, Inc.
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EGF Receptor Antibody [Sodium Azide Free], Mouse Monoclonal Antibody
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Human. Does not react with mouse and rat. Other species not known.
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Functional Activity (Inhibition): antibody blocks EGF receptor activation, inhibits A431 cell proliferation, arrests tumor growth in vivo, affinity purification<br/>IP: 1-2�g/500ug protein lysate<br/>FACS: 0.5-1ug/million cells
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188-10827-1
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RayBiotech, Inc.
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CD70 Antibody, Mouse Monoclonal Antibody
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Human. Other species not tested.
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ELISA (order Ab without BSA)<br/>Functional Activity: Inhibits T cell proliferation induced by dendritic cells (Order SAF formulation) (1)<br/>Flow Cytometry: 0.5-1ug/million cells in 0.1ml<br/>Immunofluorescence: 0.5-1ug/ml<br/>Immunoprecipitation: 0.5-1 ug/500ug protein lysate<br/>Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT
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188-10827-SAF
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RayBiotech, Inc.
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CD70 Antibody [Sodium Azide Free], Mouse Monoclonal Antibody
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Human. Other species not tested.
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ELISA (order Ab without BSA)<br/>Functional Activity: Inhibits T cell proliferation induced by dendritic cells (Order SAF formulation) (1)<br/>Flow Cytometry: 0.5-1ug/million cells in 0.1ml<br/>Immunofluorescence: 0.5-1ug/ml<br/>Immunoprecipitation: 0.5-1 ug/500ug protein lysate<br/>Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT
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RB-01-0001B-50
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RayBiotech, Inc.
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Mouse anti-bFGF/FGF basic/FGF2 [+Biotin], Mouse Monoclonal Antibody
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ELISA: By direct ELISA, at least 1:10,000 dilution will yield positive signal using HRP conjugated rabbit anti-mouse Ig (Jackson Laboratories). It also can be used as capture purpose. Western Blot: Approx 17 kDa band was observed in recombinant protein and cell lysates(A431,Human colon carcinoma tissue or COLO 320DM). Its working dilution is used at 1:500-1000 as recommended. see picture below. (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.protein marker, Lane 2-3.recombinant protein(10-1ug/lane), Lane 3-4.Normal whole cell lysate as control. The membrane blot was probed with anti-bFGF primary antibody(1 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Other applications are not tested yet. Optimal dilutions should be determined by each laboratory for each application.
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RB-01-0006B-50
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RayBiotech, Inc.
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Mouse anti-TNF alpha [+Biotin], Mouse Monoclonal Antibody
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Human TNFalpha monoclonal antibody detects protein derived from human, Jurkat cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
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Direct ELISA: antibody detection limit dilution 1:10,000-20,000, Recommended for use at 5-10 �g/ml., Western Blot: Approx 26 and 17 kDa band observed in Jurkat cell lysate. this antibody can be used at 1 - 2 �g/ml with the appropriate secondary reagents to detect human TNF-alpha.,
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130-10000B-50
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RayBiotech, Inc.
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Androgen Receptor [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa).
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130-10008B-50
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RayBiotech, Inc.
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ESR1 [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 32,000(at least detecting 32 �g/ml)), Western Blotting (recommended work dilution= 1: 1,000-5,000(at least)), (see image)Immunodetection Analysis: Lane 1.cell lysate MCF-7, Lane 2. cell lysate A431, Lane 3. cell lysate 293T, Lane 4. positive control, ER protein. The membrane blot was probed with anti-ER primary antibody (0.2 �g/ml). The binding bands were visualized using AP-conjugated goat anti-rabbit secondary antibody and then adding NBT/BCIP substrate. Arrows indicate ER in tested cell lysates (about 40 kDa).
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130-10009B-50
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RayBiotech, Inc.
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MIF [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 40,000(at least detecting 25 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein MIF, Lane 2.Jurkat cell lysate. The membrane blot was probed with anti-MIF primary antibody(0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular MIF from human and mouse cells (20 kDa).
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130-10013B-50
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RayBiotech, Inc.
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CT1 [+Biotin], Rabbit Polyclonal Antibody
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Rabbit anti-Human Cardiotrophin-1 antibody detects specifically target derived from humanrecombinant protein as well as HepG2 cell lysates. Cross reactivity with mouse and rat CT-1 was nottested yet.
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ELISA (recommended work dilution= 1:800,000(at least detecting 12.5 �g/ml), Western Blotting (recommended work dilution= 1:1000-5000(at least)), Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein CT-1. Lane 2. HepG2 cell lysate. The membrane blot wasprobed with anti-CT-1 primary antibody(0.25 �g/ml).Proteins were visualized using a goat anti-rabbitsecondary antibody conjugated to HRP andchemiluminescence detection system. Arrows indicatecellular CT-1 from human and mouse cells (24 kDa).
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130-10015B-50
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RayBiotech, Inc.
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Pro-BNP [+Biotin], Rabbit Polyclonal Antibody
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ELISA (recommended work dilution= 1: 20,000(at least detecting 50 �g/ml)), Western Blotting (recommended work dilution= 1:1,000-5,000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.recombinant protein proBNP, lane 2. T98G cell lysate. The membrane blot was probed with anti-proBNP primary antibody (0.25 �g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular proBNP from human and mouse cells (35 kDa).
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