| Applications: | SDS-PAGE |
| Species Reactivity: | Human |
| MW: | 30.3 kDa (266 aa), confirmed by MALDI-TOF. |
| Purity: | > 95% by SDS - PAGE |
| Description: | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques. |
| Further Information: | Sequence: MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN |
| Synonym: | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta polypeptide, YWHA1, YWHAH, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide, 14-3-3 eta, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide 14 3 3 protein eta, HGNC:12853, Protein AS1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide, YWHA 1. |
| Ascension Number: | Q04917 |
| NCBI EntrezGene: | NP_003396 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
