Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ERBB2-Knockout hela cells (KO, Cat# LC810070) were separated by SDS-PAGE and immunoblotted with anti-ERBB2 monoclonal antibody UM500036. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).