HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRCC (Cat# RC203805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRCC (Cat# TA812966). Positive lysates [LY416956] (100 ug) and [LC416956] (20 ug) can be purchased separately from OriGene.