HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT3 (RC215771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT3 (1:2000). Positive lysates LY403438 (100 ug) and LC403438 (20 ug) can be purchased separately from OriGene.