HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ECT2 (Cat# RC211319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2(Cat# TA807408). Positive lysates [LY413314] (100 ug) and [LC413314] (20 ug) can be purchased separately from OriGene.