HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TFF1 (Cat# RC207599, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TFF1(Cat# TA804565). Positive lysates [LY418822] (100 ug) and [LC418822] (20 ug) can be purchased separately from OriGene.