Western blot analysis of extracts from HEK293 cells transfected with N-terminal Myc-tagged gene (Lane 1, 2, 3 with 20, 10, 5uL from left to right), transfected with C-terminal Myc-tagged gene (lane 5, 6, 7 with 5, 10, 20uL from left to right) and negative control vector (lane 4) using anti-Myc tag rabbit monoclonal antibody (TA591009) at 1:2000 dilution.