HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX2 (RC200757, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX2 (T. Positive lysates LY401083 (100 ug) and LC401083 (20 ug) can be purchased separately from OriGene.