HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AAMP (Cat# RC218474, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AAMP(Cat# TA590749). Positive lysates [LY421332] (100ug) and [LC421332] (20ug) can be purchased separately from OriGene.