HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CDKN2C (Cat# RC214412, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2(Cat# TA590417). Positive lysates [LY429933] (100ug) and [LC429933] (20ug) can be purchased separately from OriGene.