HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MCM6 (Cat# RC207097, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCM6(Cat# TA590213). Positive lysates [LY401786] (100ug) and [LC401786] (20ug) can be purchased separately from OriGene.