HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DNM1L (Cat# RC221708, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNM1L (Cat# TA506153). Positive lysates [LY402138] (100 ug) and [LC402138] (20 ug) can be purchased separately from OriGene.