HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GADD45G (Cat# RC201364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45G(Cat# TA505437). Positive lysates [LY416476] (100 ug) and [LC416476] (20 ug) can be purchased separately from OriGene.