HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PGM3 (Cat# RC201522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGM3(Cat# TA504859). Positive lysates [LY414446] (100 ug) and [LC414446] (20 ug) can be purchased separately from OriGene.