HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPTN (RC210326, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPTN. Positive lysates LY413759 (100 ug) and LC413759 (20 ug) can be purchased separately from OriGene.