HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAT5A (Cat# RC205753, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A(Cat# TA502736). Positive lysates [LY418866] (100 ug) and [LC418866] (20 ug) can be purchased separately from OriGene.