HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM40A (RC210537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM40A. Positive lysates LY409738 (100 ug) and LC409738 (20 ug) can be purchased separately from OriGene.