HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GALE (Cat# RC201561, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALE(Cat# TA502146). Positive lysates [LY424739] (100 ug) and [LC424739] (20 ug) can be purchased separately from OriGene.