HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAS7 (Cat# RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7(Cat# TA501907). Positive lysates [LY418527] (100 ug) and [LC418527] (20 ug) can be purchased separately from OriGene.