HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PIM2 (Cat# RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2(Cat# TA501060). Positive lysates [LY416355] (100 ug) and [LC416355] (20 ug) can be purchased separately from OriGene.