HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY L1CAM (Cat# RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM (Cat# TA500728). Positive lysates [LY400150] (100 ug) and [LC400150] (20 ug) can be purchased separately from OriGene.