Western blot analysis of extracts of various cell lines, using Ubiquitin antibody (TA382994) at 1:1000 dilution.NIH/3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.