Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) antibody (TA382708) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 300s.