Western blot analysis of extracts of various cell lines, using Phospho-SMC1-S957 antibody (TA381769) at 1:1000 dilution. HeLa and NIH/3T3 cells were treated by UV for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 3s.