Western blot analysis of extracts of Jurkat cells, using Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb (TA380239) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.