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Ordering Details

Cat Number:TA377826
Size:100uL
Price:£387.00
Quantity:
Shipping:£12.00

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Data Sheet
MSDS

 

c Kit (KIT), Rabbit Polyclonal Antibody

Partner: OriGene Technologies

Applications:WB
Species Reactivity:Human, Mouse, Rat
Isotype:Rabbit IgG
Description:Phospho-CD117/c-Kit-Y719 Rabbit polyclonal Antibody
Shipping Conditions:Ship at ambient
Usage:Research Use Only

 

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CD117/c-Kit-Y719 antibody (TA377826) at 1:500 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CD117/c-Kit-Y719 antibody (TA377826) at 1:500 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.