Western blot analysis of extracts of various cell lines, using CD117/c-Kit antibody (TA377824) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic CD117/c-Kit .|Exposure time: 90s.