Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (TA377088) at 1:1000 dilution.NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.