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Gel: 8%SDS-PAGE<br>Lysate: 40 μg<br>Lane 1-2: A549 and HT29 cell lysates<br>Primary antibody: TA371030 (SPTLC2 Antibody) at dilution 1/900<br>Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution<br>Exposure time: 1 minute