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Gel: 8%SDS-PAGE<br>Lysate: 40 μg<br>Lane: HepG2 cell lysate<br>Primary antibody: TA351233 (GPER1 Antibody) at dilution 1/500<br>Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution<br>Exposure time: 40 seconds