WB was performed on histone extracts (15ug, lane 1 ) from HeLa cells, and on 1ug of recombinant histone H3 (lane 2) using the ab at 1:100. B. Cell cycle experiment using the ab against H3K27me3S28p. WB was performed on cells blocked in different stages of the cell cycle (1=asynchronous population, 2=G1/Sa, 3=G1/Sb, 4=S phase, 5=G2/M, 6=M, 7=G1). The picture shows a nice peak of H3K27me3S28p expression during mitosis.