WB was performed on whole cell (25ug, lane 1) and histone extracts (15ug, lane 2 ) from HeLa cells, and on 1ug of recombinant histone H3 (lane 3) using the antibody against H3K79me1. The antibody was diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.