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Cat Number:TA347133
Size:50ug
Price:£528.00
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H2A.Z (H2AFZ), Rabbit Polyclonal Antibody

Partner: OriGene Technologies

Applications:Dot, ELISA, IF
Species Reactivity:Human
Isotype:Rabbit IgG
Description:Rabbit Polyclonal H2A.Zac Antibody
Ascension Number:NM_002106
Shipping Conditions:Ship at ambient
Usage:Research Use Only

 

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Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.Zac, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the purified antibody was estimated to be 1:8, 800.

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.Zac, crude serum and flow through in antigen coated wells. The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the purified antibody was estimated to be 1:8, 800.