Western blot analysis of rat heart lysate (lanes 1 and 3) and rat liver membrane (lanes 2 and 4): 1, 2. Anti-Two Pore Calcium Channel Protein 1 (extracellular) antibody, (1:200). 3, 4. Anti-Two Pore Calcium Channel Protein 1 (extracellular) antibody, preincubated with the control peptide antigen.