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WB of human lysates showing detection of ~60 kDa TCP1-alpha protein using Anti-TCP1, clone 91a. Lane 1: MW ladder. Lane 2: A431 lysate, 20 ug. Lane 3: HEK293 lysate, 20 ug. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rat monoclonal Anti-TCP1-alpha, clone 91a incubated for 60 min at RT. Secondary antibody: Goat Anti-Rat HRP antibody (1:1000) for 60 min at RT. Colour Development: TMB solution for 5 min at RT.