Western Blot of Mouse anti-TRPC6 Antibody Lane 1: Mouse Kidney WCL Load: 10 ug per lane Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4°C Secondary antibody: DyLight™ 649 donkey anti-mouse at 1:20,000 for 30 min at RT Block: MB-070 for 30 min at RT