WB using Anti-ATDC (Ac-K116) antibody shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation.