Western blot using affinity purified anti-Pogz antibody shows detection of Pogz protein (arrowhead) in adult mouse thymus and spleen tissue lysate (Panel A). The lower molecular weight bands may be cross reactive proteins. Pre-incubation of antibody with the immunizing peptide blocks specific antibody reactivity (Panel B). Primary antibody was used at 1:20,000. Personal Communication, K.O. Gudmundsson and J. Keller, NCI, Frederick, MD.