HEK293T cells were transfected with the pCMV6-ENTRY YPEL3 (RC208686; Lane 1), CYSLTR1 (RC207763; Lane 2), SEMA3D (RC216032; Lane 3), Dmp1 (RC222208; Lane 4) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDK (1:4000).