HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY E2-Crimson (JA1132-C07, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2-Crimson (1:500).