Recombinant hGH and 20kDa hGH were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-hGH (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conj ugated to HRP and a DAB detection system. Arrows indicate recombinant hGH (22kDa) and 20kDa hGH, respectively.